Dear Colleagues,
has anyone grown / produced in insect cells in SILAC medium (Arg- Lys -)?
Thanks
Best
Sabine
Hi Sabine,
this is described in the paper of Nuno Carinhas from the group of Paula Alves.
They use Sf900II without arg and without lys. The method is described in
doi:10.1371/journal.pone.0026444
We have no experience with the SILAC method in Insect cells but they can help you for sure.
Sincerely yours Joop van den Heuvel