The Cellular Imaging platform allows the access to a confocal microscope (spinning disk S-M4D), a video-microscope (V-M4D), a super-resolution microscope (SMLM SR-M4D) and a flow-cytometer (VYB). The instruments are optimised to study living cells, and fluorescent protein expression. Details are available on the ISBG website.
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login to contact The spinning disk confocal microscope (S-M4D; Olympus and GATACA) has been upgraded in May 2022. It is configured with a Nipkow disk (Yogokawa CSU-X1) and 6 solid state lasers for confocal imaging in real time on living cells (very low phototoxicity). Acquisition is performed using an EMCCD camera (Andor iXon ultra ; MetaMorph). A photoconversion/photoactivation module is available for FRET and FRAP (Ilas2).
The time-lapse video-microscope (V-M4D, Olympus), renewed in 2019, is composed of an inverted Olympus IX83 fully motorized microscope equipped with a blind, temperature controlled, incubation chamber (Digital Pixel) for live imaging. Acquisition is achieved with a sCMOS camera (Hamamatsu Orca Flash4; CellSens).
The multi-channel flow-cytometer (VYB, Miltenyi biotech) is equipped with three lasers and 8 fluorescence channels. It is optimised for fluorescent proteins, and covers all the emission wavelengths used with microscopy. It is automatable (multiwell plate, immuno-staining, dilutions), and compatible with magnetic columns Miltenyi (isolation of rare events).
Cytometry and microscopy analysis software are available for users in a dedicated room. Three workstations are installed with Volocity and Imaris for microscopy data visualization and analysis; or MacsQuant and CellQuest for flow cytometry.