Electron Microscopy and Cryo-CLEM, CNB-CSIC, Madrid, Spain

The facility will provide support and expertise to researchers using different techniques: Cryo-EM sample preparation and characterisation, Cryo-EM image acquisition of unstained biological material for cryo-EM single particle or cryoelectron tomography (cryo-ET), cryo-electron diffraction (microED) and cryocorrelative microscopy (cryo-CLEM). For many of these services, it is advisable to contact the Instruct Image Processing Centre for data processing support).

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CryoEM CNB-CSIC

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User Guide

In order to provide you with our best services, we require the following information:

It is extremely important to contact the facility (cryoemcsic_facility@cnb.csic.es) before submitting the proposal, to discuss the technical feasibility of your experiment and discuss the details on how it will be carried out.
Molecular mass of the sample (protein or complex)
List the buffer conditions of the sample solution.
If the sample contains a reducing agent, glycerol, imidazole or detergents, a further contact with the facility may be necessary (cryoemcsic_facility@cnb.csic.es)
Protein concentration of the sample must be between 1-3 mg/ml
Several aliquots must be sent (e.g. 4 X 20 µl), together with 20 ml of the buffer solution. In the case of the buffer containing detergent, send the buffer with and without detergent.
The sample must be sent at the temperature in which is best kept.
In case data are going to be acquired, the user must be responsible of their storage. It is always advisable to contact the facility regarding this matter for further advice.