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Electron Microscopy, CSSB, Hamburg, Germany

The facility provides access to state-of-the-art cryo-EM instrumentation, including automated workflows for single particle data analysis, electron cryo-tomography, lamella preparation, and correlative imaging.

The cryo-EM facility offers the following workflows:

  • Sample preparation and Cryo-grid screening on the Arctica for samples prepared on Quantifoil grids (e.g. single particle analysis targets, exosomes, vesicles), including 2D classification for initial quality assessment.
  • High-end single particle data collection on the Titan Krios with cryoSPARC Live processing for pre-screened grids on the Titan Krios.
  • Tomogram acquisition on pre-screened samples such as large complexes, exosomes, and viruses.

Tailored support is provided for Instruct users, including project feasibility assessments, proposal preparation, and scheduling support.

View All at Instruct

 

Instruct Centre

Instruct-Hamburg (DESY, CSSB, European XFEL)

Service/Technology Contacts

User Guide

Instrumentation available:

 

  1. Talos Arctica Cryo-TEM

Used for sample screening (primarily purified protein complexes, vesicles), grid evaluation, EPU multigrid and cryoSPARC for fast evaluation. Tomography on thin samples is possible.

Physical access: Yes

Remote access: Yes (supervised sessions possible)

Software: Velox, EPU, TOMO

 

  1. Titan Krios G3 (Thermo Fisher Scientific)

High-end cryo-TEM for data collection of single-particle or tomographic tilt-series datasets at highest resolution.

Physical access: Yes

Remote access: Yes (under supervision)

Software: MAPS, EPU, TOMO or SerialEM, cryoSPARC live

 

  1. Leica GP2 Plunger

Used for sample vitrification.

Physical access: Yes

Remote access: No

 

  1. Vitrobot

Used for sample vitrification.

Physical access: Yes

Remote access: No