The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM). The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis.
login to contact
login to contact
login to contact
login to contact Access to the following instrumentation is provided: the Glacios cryo-electron microscope which allows high-resolution SPA and CET automated data collection and two Titan Krios cryo-electron microscopes with CMOS high-sensitivity direct electron detector camera. The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV.
The Titan Krios is the latest generation electron microscope with capacities for high-resolution data collection for both Cryo Electron Tomography (CET) experiments and Single Particle Analysis (SPA) with automated data collection. The electron source is a Field Emission Gun (FEG) that can be operated at 80keV, 100keV, 200keV or 300keV. An automatic loading mechanism allows mounting twelve grids at a time.
The Titan 1 microscope is equipped with CMOS (FEI FALCON3 4K*4K and GATAN K3) high-sensitivity direct electron detector cameras, corrector CS, phase plate and GIF.
The Titan 2 microscope (G4) is equipped with Selectris X energy filters and Falcon 4i camera.
The Glacios cryo-electron microscope allows high-resolution SPA and CET automated data collection. Its FEG is usually tuned to 200keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with two digital cameras: FEI Ceta-D and the direct electron detector GATAN K2
The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV. Grids are mounted one at a time using a Gatan side-entry cryo-holder. This microscope is equipped with one digital camera (GATAN CCD 2K*2K "US10001"), it is used to collect data for cryo-SPA or room temperature electron tomography using a Fischion side-entry holder. Cryo-EM grids are flash-frozen in a temperature and humidity controlled environment using a Vitrobot system (FEI).
All the microscopes are equipped with digital cameras, images can be recorded as films and scan them with a high-resolution Heidelberger Druckmaschinen drum scanner (5 micron pixel size). When asking access for Titan or Glacios, please add to your proposal preliminary image of the sample in cryo condition.