HDX Mass Spectrometry gives information of the exchange of hydrogen for deuterium on the protein backbone. Uptake rate is determined by each site’s solvent accessibility. Comparing the extent of deuterium exchange in a ‘apo’-protein with a target (agonist, antagonist, inhibitor, lipid etc) bound protein can provide information about conformational changes, active sites and important binding residues.
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Please contact us before applying for an HDX visit to discuss the appropriateness of the method. Depending on the complexity of your samples, it might be necessary to apply for two visits.