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ISPC (WIS) Israel

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User Guide

For crystallisation trials we will need a sample which is:

• Pure as assessed by SDS-PAGE
• Homogeneous i.e. devoid of truncated proteins, isomorphs, or incorrectly folded molecules 
• A reproducible source of several milligrams of the pure protein
• The protein should be at an optimal concentration, in the range of 10-40mg/ml, as determined in a pre-crystallisation trial by the crystallisation unit. The salt concentration should be as low as possible, the buffer concentration should be 20-50mM, at a pH close to neutral, and potassium should be avoided if possible
• For each of 96 crystallisation conditions (one plate regardless of the crystallization method used) 25μl of protein is needed. 150μl of protein is needed for initial screening