Lipidic cubic phase (LCP) crystallisation is an alternative to standard crystallisation for membrane proteins. It allows membrane proteins to crystallise in a close to native and stable environment, in a lipidic bilayer. G-protein-coupled receptors (GCPR) protein crystallisation demonstrated the power of this approach; Cherezov V et al. High Resolution Crystal Structure of an Engineered Human β2-Adrenergic G protein-Coupled Receptor. As LCP is described as infinite 3D periodic structure, membrane proteins (MP) can freely diffuse laterally along the membrane. Diffusion is one of the important pre-crystallisation characteristic of the MP embedded into lipidic bilayer, which could be checked by Fluorescence recovery After Photobleaching (FRAP) technique available at HTMPC platform.
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