The HT Robotein® facility at CIP and SBBC has the particularity to combine high technology laboratory instrumentation: a central liquid handling workstation, a colony picking instrument, various microplate spectrophotometers, an Octet HTX, a LabChip GXII, a protein spotter and an Infrared Imager. This specific combination of equipment offers versatile tools for the development of numerous HT applications. Here is a list of the main services that Robotein® can provide. Nonetheless, we are always open to develop new and innovative assays.
We have developed well-established protocols for automated transformation of various bacteria and yeast strains, and for site-directed or random mutagenesis. Our platform allows automated cell cloning, high throughput colony picking, as well as cell line generation. Following automated colony picking, cultures in DeepWell plates allow selection of the best producers, together with screening for optimal culture medium. The highest expression yields can be accurately and rapidly tested on the LabChip GXII and/or the Octet HTX instruments.
Expressed proteins can be purified in a 96-well format on the robotic workstation. Automation of this process increases throughput at each purification step, reduces human error and results in higher reproducibility with an increase in yield. The efficiency of the different purification steps can be assessed on the LabChip GXII and/or the Octet HTX equipment.
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