CERM/CIRMMP offers unique research capabilities in the field of solid-state NMR of biomolecules by providing state of the art instrumentation and expertise to perform, at the highest level, the most comprehensive array of experiments needed for the structure and dynamic characterisation of biological macromolecules and their complexes.
Solid-state NMR available at CERM/CIRMMP can be used to obtain atomic-level structural information of biomolecules when they are forming aggregates (e.g.: fibrils), or when they are bound to or trapped in solid matrices that lack long-range three-dimensional order (e.g.: immobilized enzymes), or even for those systems that are too large and escape the conventional limits of NMR in solution.
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login to contact Solid State NMR spectroscopy measurements can be performed on virtually any typeof biological solid. CERM/CIRMMP has experience of working with several different kinds of biosolids including microcrystalline proteins, amyloids, fibrils, biomaterials and sedimented solutes.
To obtain structural information on proteins by solid- state NMR, 15N/13C isotopically labelled samples are usually required. Deuteration may also be necessary when using 1.3 mm rotors to perform 1H-detected experiments.
Thanks to recent upgrades, our 1.2 GHz spectrometer is now equipped with a 0.7 mm solid state MAS Probe. Ultra-fast magic angle spinning at 1.2 GHz allows to record high quality 1H-detected spectra with outstanding resolution, without the need of deuteration, enabling to monitor dynamics and interaction at the solid-state. Users can thus perform the following studies: assignment, determination of structural constraints; local dynamics; protein-protein and protein-ligand interaction; determination of paramagnetic effects.
The support might include instruments setup, data acquisition and data analysis. Assistance and supervision will be assigned based on the expertise of the visiting scientist.
In case of remote access, the transfer to the rotor is performed by our staff only for 3.2- and 4-mm rotors and non-air/light sensitive samples. For high MAS frequencies (rotors of 1.3 mm or 0.7 mm), samples must be provided directly in the rotor, after testing that they spin properly.
The visiting scientist must communicate the scientific plan of the experiments and the contacts to arrange the visit. On average a visit is one week of instrument time and it can be arranged as a physical visit to the facility or by mailing-in the samples. Some projects might require more time, up to several weeks, depending on the complexity of the work to be performed and the experience of the visiting scientist in the field.
Contact the facility staff before submitting your proposal, so that the access request is in agreement with the scientific plan of the experiments.