X-ray Diffraction and Bio-SAXS laboratories are a part of Biomolecular Interactions and Crystallography core facility (BIC) at CEITEC Instruct centre. The laboratories are equipped with top-class instruments for diffraction experiments with single crystal samples focused on the determination of the 3-D structure of (macro)molecules down to atomic resolution and for small angle X-ray scattering experiments with isotropically scattering samples focused on determination of the shape and size of macromolecules or nanoparticles.
Services include diffraction/SAXS data collection, analysis and structure determination.
More information can be found on the Biomolecular Interactions and Crystallography core facility webpage.
login to contact
login to contact The range of applicable molecular mass for diffraction methods: from 102 up to 106, where the lower value covers molecules significant for nanotechnology, materials science or pharmacology and the upper limit covers bio-macromolecules such as nucleic acids, proteins and their complexes.
Range of applicable particle sizes for SAXS: from 2 to 100 nm.
For both, diffraction and SAXS, samples could be delivered in person or via delivery service as a mail-in access.
The diffraction of X-rays in single crystal samples is the most important and – if an appropriate sample is available – also the fastest methodology currently available for the determination of the atomic structures of molecules and/or macromolecules and their complexes.
On the other hand, SAXS is a technique capable of determining structural characteristics such as mono dispersity or aggregation, oligomeric state, low resolution three-dimensional shape or even quaternary structure not from a crystal but from a solution of (bio) macromolecules.
The centralised organisation of expensive instrumentation for SAXS and single crystal studies and highly trained staff allow the cost-effective use of resources and obtaining of experimental results in rapid responses to the demands of even untrained users.